Table 2 Comparison of several fluorescence microscopy in imaging [12]
From: Intravital microscopy imaging of kidney injury and regeneration
Property | Two-photon microscopy | Standard fluorescence microscopy | Laser scanning confocal microscopy |
|---|---|---|---|
Imaging depth | < 1000 μm | All (out of focus) | < 500 μm |
Effective resolution | High | Low | Low |
Photo bleaching | Low | Medium~high | Medium |
Excitation | Limited to objective focal volume | All | Entire specimen in objective focal axis |
Emission | Dependent on specimen and image depth | N/A | Dependent on specimen and image depth |
Main limitations | Imaging time, heat, price | Imaging depth, Z-resolution | Imaging time, photo damage |
Main advantages | Imaging depth, low photodamage | Accessibility very good for 2D of thin sections, additional deconvolution | Accessibility, 3D imaging |
Cost | Very high | Low~high | High |