Fig. 1

Differentiation protocol for inducing human iPSCs into OSR1⁺SIX2⁺ renal progenitors. The differentiation method used to generate OSR1⁺SIX2⁺ renal progenitors with embryoid body (EB)-based 3D cultures. CHIR99021 an inhibitor of glycogen synthase kinase 3 (GSK3), TTNPB an analog of retinoic acid, DMH1 a selective BMP antagonist, FBS fetal bovine serum, P/SM penicillin streptomycin, KSR knockout serum replacement, 2ME 2-mercaptoethanol, NEAA non-essential amino acid, BMP bone morphogenetic protein, TGF transforming growth factor. Adapted from Toyohara et al. [41]