Intravital microscopy imaging of kidney injury and regeneration

Table 1 Some common fluorescent molecules for two-photon excitation [57, 62,63,64,65]

Application	Fluorophores	2P excitation (nm)	Emission (nm)
Membrane probe	DiI	830~920	565
	DiO	780~830	510
	Filipin	720	510
Nucleic acid stains	Hoechst	780~820	455, 478
Nucleic acid stains	PI	820~850	617
Mitochondrion probe	Mito Tracker red	750~840	600
Fluorescent proteins	eYFP	860~950	532
	eGFP	820~950	509
	eCFP	800~900	476
	eBFP	800	445
	tdTomato	760~800	581
	mCherry	750	610
Conjugates dyes	CY2	780~800	506
	CY3	780	565, 615
	CY5	780~820	670
	FITC	740~820	519
	Rhodamine 123	780~860	550
Conjugates dyes	Alexa 350	750~800	440
	Alexa 488	720~800	515
	Alexa 516	720~840	569
	Alexa 568	720~840	596
	Alexa 594	720~860	580
	Alexa 633	720~900	647
	bis-MSB	680~750	420
Neural tracer	Bodipy	900~950	512
	DiD	780~820	670
	Lucifer Yellow	860~890	533

DiI 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate, DiO 3,3′-dioctadecyloxacarbocyanine perchlorate, PI propidium iodide, YFP yellow fluorescent protein, GFP green fluorescent protein, CFP cyan fluorescent protein, BFP blue fluorescent protein, FITC fluorescein isothiocyanate, DiD 1,1′-Dioctadecyl-3,3,3′,3′-Tetramethylindodicarbocyanine,4-Chlorobenzenesulfonate salt

ISSN: 2059-1381