Table 3 Imaging technologies promote tissue-wide lineage tracing studies
From: Intravital microscopy imaging of kidney injury and regeneration
Imaging technologies | Transgenic mice | Outcome | Ref/ |
|---|---|---|---|
Bioluminescence imaging (BLI) | Ngal-Luc | Illuminated injuries in vivo in real time | [82] |
Multiphoton microscopy (MPM) | Podocin-GFP; Podocin-confetti; PEPCK-GFP | Visualize the motility of podocytes and parietal epithelial cells (PEC) in vivo | [83] |
Confocal microscopy | Coll1α-GFP-CreERT2; R26Lacz; R26mTmG; R26Tomato | Easily detect anatomic features of podocytes | [84] |
Multiphoton microscopy (MPM) | Pod/Cre-GCaMP3/fl | Reveal the importance of podocytes Ca2+ in glomerular pathology | [85] |
Two photon microscopy (TPM) | Confetti/Podo:Cre; CA-Rac1/Nphs1-rtTA | Podocytes change from a static to a dynamic state in vivo | [86] |
Two photon microscopy (TPM) | GFP-CaMP2 | Detect basic calcium levels in proximal tubular epithelial cells | [87] |
Multiphoton microscopy (MPM) | PDGFRß-CreERT2; R26-mTmG | PDGFRβ mediates the communication between the renal interstitium and the tubule system | [76] |
Two photon microscopy (TPM) | Sox9-CreERT2; R26-mTmG | In vivo imaging of the fate of green Sox9+ cells | [5] |
Two photon microscopy (TPM) | mRen-Cre; R26-mTmG | The niche of progenitor cells of the renin lineage cell is continuously filled by the neonatal differentiation of the kidney | [88] |
Multiphoton microscopy (MPM) | Ren1c-Cre; R26-Confetti; Ren1d-Cre; R26-Confetti | In focal segmental glomerulosclerosis, renin lineage cells migrate to the glomerulus and replace podocytes and PECs | [89] |