Fig. 2

In vitro evaluation methods. A Experiment 1 method. The arterial circuit was connected to a water reservoir, and the vein circuit was connected to a tank for waste fluid, creating a system with a flow rate of 150 ml/min. For visualization of the flow, 1 ml of dye was injected into the venous air trap chamber. The time period of dye disappearance from within the venous air trap chamber was measured after dye injection, using visual evaluation. B Experiment 2 method. An experimental system was constructed in which both the arterial circuit and vein circuit were connected to a water reservoir, with a recirculating flow at a rate of 150 ml/min. The circulating fluid was a glycerin solution mixed with silver dye, and the fluid movement in the chamber was visually monitored