Skip to main content

Dialysis efficacy and pain levels in relation to cannulation distance using the buttonhole technique



In this study, we investigated whether there was any difference in pain and dialysis efficacy when patients who initially entered dialysis with the rope ladder technique were switched to the buttonhole technique.


We reduced the space between needles and examined the effects on access blood flow rate, dialysis efficacy, and pain levels with BH technique. Forty-four patients participated, 25with dialysis needles placed 5–7 cm apart using the BH technique, and 19 with needles placed 7–10 cm apart.


There was a significant reduction in the pain sensation in both groups from patients who passed the rope ladder technique to the buttonhole technique from the onset to the sixth month. There was no difference in dialysis efficacy between the patients who passed the lope ladder technique to the buttonhole technique; also, there was no difference in the dialysis efficacy compared to the needle entry interval in two groups of patients who were dialyzed with the BH technique.


The BH technique continues to attract interest due to its dramatically reduced pain levels compared to the RL technique and no difference in dialysis efficacy.


It is essential to establish and maintain appropriate vascular access in haemodialysis (HD) patient [1]. Arteriovenous fistulae (AVF) are recommended by many national clinical guidelines as the vascular access of choice for HD patients with end-stage kidney disease [2]. The traditional type of AVF puncture is the rope-ladder (RL) technique. Another method, the buttonhole (BH) or constant-site technique, uses the same AVF cannulation sites for every dialysis session with the aim of minimising venous damage [3]. The BH puncture method is considered a good alternative to RL and is currently recommended by several scientific societies, including the National Kidney Foundation/Kidney Disease Outcomes Quality Initiative (NKF/KDOQI) and the European Vascular Access Society [4]. BH needling was initially popular for home HD, but its use has spread to include clinical centre dialysis. The technique requires the creation of a single tract using a standard sharp fistula needle, which can then be accessed using a blunt needle at the same entry site and tract each time. The main advantages reported for the BH technique include easier AVF cannulation, fewer puncture failures, less pain, more rapid haemostasis after needle removal and fewer aneurysms and haematomas [5]. However, several studies have shown a higher rate of infection associated with BH fistula access compared to sharp needle puncture techniques [6]. Recent systematic reviews have concluded that BH cannulation may be associated with a higher rate of local infection and bacteraemia compared to area puncture needling, but more definitive studies are required to reach firm conclusions [7, 8], as some studies have not noted increased systemic infections from BH needling [9].

Observational studies have failed to prove a longer fistula survival time for the BH technique compared to standard RL needling [9, 10]. In this study, we wanted to compare the cannulation pain levels and dialysis activities between the rope ladder technique and the buttonhole technique. We also distinguished the patients using the buttonhole technique, two groups according to the distance of the cannulation points. We compared their pain values and dialysis activities.

We investigated several parameters, such as vascular area blood flow rate (BFR) between the needles, dialysis venous pressure (DVP), dialysis adequacy (Kt/V), pain during cannulation and the distance between needles. Patients with AVF were placed into two groups according to entry points. We compared patients with fistula needles spaced from 5–7 cm to those with spaced from 7–10 cm in terms of pain sensitivity and dialysis efficacy.

Material and methods

This study was approved by the Baskent University Institutional Review Board and Ethics Committee (project no: KA15/170). The research adhered to the tenets of the Declaration of Helsinki, and a detailed written informed consent form was obtained prior to each individual’s participation in the study. We designed a prospective single-centre crossover study to compare the BH and RL cannulation methods for 1 year in 44 stable HD patients with end-stage renal disease undergoing regular HD in the Haemodialysis Unit at Baskent University Konya Hospital. Eligible individuals were over 18 years of age and had received HD three times weekly, 4 h per session, for at least 6 months prior and had mature AVFs used for RL cannulation.

In the study, BH tunnel tracts were established for all the patients. Three patients were excluded from the study due to prolonged bleeding in the fistula whilst starting the buttonhole cannulation. In patients undergoing the RL technique in previous sessions, a tunnel line was created for the BH technique by the selected nurses at the beginning of the study which were experienced in cannulation access. Pain sensitivity and dialysis efficacy were investigated according to the distance between cannulation points after the tunnel was established. Haemodialysis patients who had no problem with their fistulas were included in the study. All patients understood the purpose of this study, and there were no problems with communication. Using local anaesthetic agents during cannulation might reduce the pain, but it was not cost effective. The patients were informed about the study and their approval was obtained. The exclusion criteria were AV grafts or central venous catheters, an infection on the fistula line, skin disease over or around the access point, a need for interventions due to AVF dysfunction, and the inability to tolerate > 300 ml/min of blood flow during HD. The blood flow rate (BFR) of the HD machine was maintained at 300 ml/min, and the perfusion rate of the dialysis solution was 500 ml/min. Heparin was used for anticoagulation during HD.

To form the tunnel line, six to nine cannulations were made by the same nurse for the same patient. The skin at the cannulation site was cleaned with alcohol and betadine, and two 16-gauge sharp needles were inserted at 20–45° angles [11]. The arterial cannulation site was placed in the more distal segment of the access but at least 3 cm from the AV anastomotic site. A venous needle was inserted at a minimum distance of 5 cm proximal to the arterial needling site to avoid the previous cannulation site.

The patients were divided into two groups according to the distance between the arterial and venous needle points. The diameter of the area between the arterial and venous cannulation sites was 5–7 cm in 25 patients (group 1) and 7–10 cm in the remaining 19 patients (group 2). After six to nine cannulations with sharp needles, two pairs of tunnel tracts for the BH method were established at the venous and arterial needling sites [12]. After six to nine dialysis sessions, the same nurse inserted 16-gauge blunt needles at 25° angles for the BH technique. Needles were inserted using the classical method, with the arterial set directed towards the extremity of the arm, and the venous set directed towards the heart. The distance between the needles was measured with a ruler.

Vascular access BFR in the area between the needles (AVF BFR), Kt/V and DVP were measured during the first week and at the end of the 12th month study period. All patients underwent colour Doppler examinations with a EUP-L535 (Hitachi, Tokyo, Japan). Ultrasonography was performed with the patients in the supine position at 7.5 MHZ (Hitachi, EZU-MT 24-51, Tokyo, Japan) during haemodialysis. Silica gel was placed on a superficial probe, and the Doppler velocity was measured. Speed measurements were made through the dialysis needle entry points at appropriate angles. The normal range of vascular access flow (BFR) was defined as 600–1200 ml/min [5]. DVP was measured within 5 min after starting dialysis to prevent ultrafiltration effects. The normal DVP range was defined as < 100 mmHg. DVP values over 100 mmHg or a gradual increase suggested recirculation [11]. As an indicator of dialysis efficiency, Kt/V was measured using a single-pool urea kinetic model [13]. The normal range was defined as ≥ 1.4 according to the NKF-KDOQI guidelines [13]. Pain associated with needle insertion was measured at baseline, 6 and 12 months using a 10-point visual analogue scale, with 0 indicating no pain and 10 indicating severe pain.

Kt/V was measured at baseline and at the 12th month. The HD dose is quantified by Kt/V, which measures urea removal during treatment, and a single-pool KT/V of 1.2 is considered an adequate dose [14]. The primary data from the National Cooperative Dialysis Study showed that the Kt/V of < 0.8 was associated with high mortality, whereas Kt/V values between 1.0 and 1.2 were associated with better outcomes [15]. El-Sherikh et al. found that low recirculation percentages result in improved dialysis effect. The differences in the Kt/V values amongst these recirculation groups were statistically significant [16].

Statistical analysis

Statistical data were analysed using SPSS version 21.0 (SPSS, Chicago, IL, USA). Values were expressed as mean ± standard deviation. The normality of the values was analysed using Kolmogorov-Smirnov test. Student’s t test was used according to Kolmogorov-Smirnov test results. Differences were considered significant at p < 0.05. Correlations between the variables were investigated based on Pearson or Spearman correlation coefficient.


The mean age of the patients in this study was 66.6 years, and 50% were men. The distribution of the patients according to disease aetiology was as follows: 18 with diabetic nephropathy (40.909%), 15 with hypertensive nephropathy (34.09%), six with glomerulonephritis (13.63%) and five with amyloidosis (11.36%). The mean duration of AVF access was 38.09 months. As expected the average pain in the BH technique was less than the RL technique. There was a significant difference between the three pain scale measurements at 0, 6 and 12 months (p < 0.05, multiple variance analysis). There was a significant decrease in pain measurements from 0 month (pain measurement of 6.5682/10) to 6 months (pain measurement of 4.5909/10), to 12 months (pain measurement of 3.2727/10) (p < 0.05, paired t test) (Fig. 1).

Fig. 1
figure 1

Time course of pain scale: first (0), second (6) and third (12th month) pain levels (change in pain level when switching from robe ladder technique to buttonhole technique)

The Kt/V(1) value at the beginning of the study was calculated using the rope ladder technique. At the end of the study, the Kt/V(2) value was calculated based on the buttonhole technique.

There was no difference between the first (1) and second (2) measurements in terms of dialysis efficacy, with a Kt/V(1) of 1.48 ± 0.33 and a Kt/V(2) of 1.49 ± 0.22 (p = 0.82, paired t test). There was no correlation between Kt/V values for the first and second measurements relative to pinhole distance. The Kt/V values of the first measurement using the RL method and the last measurement using the BH method were not significantly different (Fig. 2). The distance between needle punctures was significantly shorter in the diabetic patients: 1.3 ± 0.47 compared to 1.61 ± 0.5 in non-diabetic patients (p = 0.047, Student’s t test).

Fig. 2
figure 2

Percentage of Kt/V urea according to needle-puncture distance of patients entering dialysis in BH technique. Change in Kt/V value according to cannulation distance in buttonhole technique (p = 0.82)

The measurement of blood flow velocities in the AV fistula areas of the patients were checked before and at the end of the study. There was a significant difference between Doppler 1 and 2 measurements in blood flow velocity in the area between the drainage holes: Doppler 1 = 95.56 ± 36.43, Doppler 2 = 106.86 ± 37.94 (the change in needle puncture distance was correlated with the effects of Doppler 1 and 2 (p < 0.05, paired t test). There was a positive correlation between the Doppler flow difference in the cannulation area BFR and DVP (p = 0.009, r = 0.387, Pearson’s correlation test) (Fig. 3). Cannulation distance had a significant effect on the BFR difference (multiple variance analysis, p = 0,031). As the cannulation distance decreased, the flow velocity in the Doppler increased. This difference can be explained by the increase in recirculation as the cannulation distance decreases. This difference is shown in Fig. 3.

Fig. 3
figure 3

The relationship between needle puncture distance and BFR (blood flow rate—ml/min) in the cannulation area. Change in BFR by cannulation distance over the course of the study (p < 0.05)


Research on long-term BH cannulation has shown that pain is reduced over time using this technique. Pain is the most common complaint of HD patients during RL cannulation using sharp needles [13]. It is the main reason for refusal to undergo dialysis, and it reduces the patient’s quality of life. The success of arteriovenous fistula cannulation is dependent on many variables. One of these variables includes the length of vascular access available to accommodate two needles [17]. Effective haemodialysis is directly related to a better health condition, lower morbidity and mortality rates for haemodialysis patients. But a good quality of dialysis is provided by a series of factors, amongst them the technique of AVF puncture and the amount of blood recirculation. Blood recirculation occurs when the rate of access flow becomes lower than the rate of dialyzer blood flow especially in the presence of inadequate location of the needles inserted into the AVF [18]. BH cannulation has been shown to be a simple and cost-effective alternative for reducing pain compared to RL cannulation [19]. One observational study showed the BH technique to be associated with an increased perception of pain; van Loon et al. showed that the average pain score in patients undergoing the BH technique was greater than patients undergoing the RL method [5], these findings are not consistent with other studies, including the present one, in which pain was decreased with BH cannulation compared to the RL method, with significant differences between the three pain-scale measurements (p < 0.05). In contrast to the studies showing that the BH technique is less painful, findings were obtained in two randomised studies. Vaux et al. and Mac Rae et al. attributed more pain in the BH technique to the application of home haemodialysis and more frequent cannulation [20, 21]. Additionally, whilst the distance between the needles should be a minimum of 10 cm for cannulation in the RL technique, there is no such inquirement in the BH technique [5]. For patients with a very low pain threshold, the BH technique can be an attractive alternative. However, there was also a significant difference between the pain levels (p < 0.05), suggesting that pain gradually diminished when a patient was switched to the BH technique after being previously dialysed with the RL technique (Fig. 1).

In our study, patients originally underwent HD using the RL technique and then received HD with the BH technique for 1 year. At the end of this period, there was no difference in dialysis efficacy between the two cannulation techniques. The patients who received dialysis with the BH technique were also divided into two groups according to pinhole distance, with fistula needle spacing of either 5–7 cm or 7–10 cm. Kt/V ratios were compared between these two groups at baseline and at 12 months, and there was no significant difference in terms of dialysis efficacy over time (p = 0.82) (Fig. 2).

A similar study was performed by Rothera et al., who showed that an AVF can be cannulated with needles 2.5 cm apart, with no cases of access recirculation [17]. We planned the present study differently, using patients entering dialysis with the BH technique, as studies on tunnelled patients could not be found in the literature. Dias et al. investigated fistula cannulation techniques and found that needles placed unidirectionally and < 5 cm apart were associated with more access recirculation and lower Kt/V than with other combinations; the arterial set directed towards the extremity of the arm and the venous set directed towards the heart was the preferred technique for needle insertion at a distance of ≥ 5 cm [18].

For each HD treatment, the fistula is cannulated, usually with two needles. The arterial needle allows the blood to be withdrawn from the patient into the dialysis circuit, and then it is returned through the venous needle. Studies generally state that there should be a minimum of 5 cm between these needles to prevent or minimise blood access recirculation in order to provide efficient HD treatment [17]. The success of AVF cannulation is dependent on many variables, including the length of vascular access available to accommodate two needles. Therefore, if the fistula cannulation distance is, for example, 2.5 cm, an alternative access route for dialysis can be sought.

Santoro concluded that the form of needle insertion and the distance between the needles should be considered in the process of recirculation reduction, with the classical form providing the lowest percentages of access recirculation and with unidirectional needles providing satisfactory results with a ≥ 5 cm distance between them [22]. The BH technique is generally easier than RL and is widely used in home dialysis where self-cannulation is performed, and can only be used for native AVF [23].

Kim et al. showed that there were no statistical differences in vascular access BFR, DVP and Kt/V between the RL and BH methods; however, cannulation pain was decreased with BH compared to RL [24]. Kt/V values using the RL method at the beginning of their study and in the last week using the BH method were not significantly different; this result is similar to our findings (Table 1).

Table 1 Descriptive statistics. Kt/V and needle distance

When we measured Doppler flow in the area between needle entry points, the initial measurements were higher after 12 months, and the Doppler BFR in the area between the needles increased over time. There was a positive correlation between Doppler BFR and DVP (Fig. 3).

The correlation between DVP and blood flow velocity can be interpreted as the risk of recirculation increasing with decreased fistula needle distance. Consistent with previous studies [18], recirculation may occur in AVFs as this distance becomes shorter. In our study, the cannulation points and the distance between the needles had significant effects on Doppler flow velocity; as needle distance decreased, blood flow velocity in the region increased. Although there was no difference in efficacy when transitioning to the BH technique, pain levels were dramatically reduced in the present study, more research is needed on the BH technique, as it seems to increase the risk of recirculation (Table 2).

Table 2 Comparison of demographics and comorbidities of the study population

This study had some limitations. It was carried out in a single centre. In addition, we only observed ideal, well-functioning AVFs that were easily cannulated by nurses who were experienced in using these cannulation techniques. BH is useful in patients with AVF in whom multiple cannulation sites are absent. Therefore, the fistula entry points of all patients were set to be between 5-10 cm and healthy fistulas that were not experiencing any problems before included in the study.

Cannulation of the AVF in today’s HD population is more difficult and abrasive than ever.

Our study has shown that the BH technique may be preferred because it is less painful, does not differ from the RL method in efficacy and can be applied to shorter vasculature.

Successful and painless fistula cannulation requires the dialysis nurse to be experienced and skilled. Quality and effective dialysis services in terms of patients bring patient satisfaction. If all these conditions occur, we think that the dialysis patient will be easier to adjust and haemodialysis treatment will be more successful.


The BH technique continues to attract interest due to its dramatically reduced pain levels compared to the RL technique and no difference in dialysis efficacy. We think that this technique is especially important for patients with vascular problems, as the same entry points are always used in the buttonhole technique. We believe that different studies are needed to assess different cannulation distances in terms of pain and efficacy in these patients.

Larger, more definitive studies are needed to determine whether this technique is safe for broader use.

Availability of data and materials

Please contact the authors for data requests.





Arteriovenous fistulae






National Kidney Foundation/Kidney Disease Outcomes Quality Initiative


Blood flow rate


  1. Knatole B, Deborah B. Improving arteriovenous fistula construction: fistula first initiative. Hemodial Int. 2004;8(3):199–206.

    Article  Google Scholar 

  2. Fluck R, Kumwenda M. Renal association clinical practice guideline on vascular access for haemodialysis. Nephron Clin Pract. 2011;118(Suppl 1):225–40.

    Article  Google Scholar 

  3. Twardowski Z, Lebek R, Kubara H. 6-year experience with the creation and use of internal arteriovenous fistulae in patients treated with repeated hemodialysis. Pol Arch Med Wewn. 1977;57(3):205–14.

    CAS  PubMed  Google Scholar 

  4. NFK-KDOQI Clinical Practice Guidelines and Clinical Practice Recommendations. 2006 Updates. Available at Accessed 6 Jan 2016.

  5. Van Loon MM, Goovaerts T, Keessels AG, Van der Sande FM, Tordoir JH. Buttonhole needling of hemodialysis arteriovenous fistulae results in less complications and interventions compared to the rope-ladder technique. Nephrol Dial Transplant. 2010;25(1):225–30.

    Article  Google Scholar 

  6. Macrae JM, Ahmed SB, Hemmelgarn BR, Alberta Kidney Disease Network. Arteriovenous fistula survival and needling technique: long-term results from a randomized buttonhole trial. Am J Kidney Dis. 2014;63(4):636–42.

    Article  Google Scholar 

  7. Wong B, Muneer M, Wiebe N, Storie D, Shurraw S, Pannu N, et al. Buttonhole versus rope ladder cannulation of arteriovenous fistulas for hemodialysis: a systematic review. Am J Kidney Dis. 2014;64(6):918–36.

    Article  Google Scholar 

  8. Grudzinski A, Mendelssohn D, Pierratos A, Nesrallah G. A systematic review of buttonhole cannulation practices and outcomes. Semin Dial. 2013;26(4):465–75.

    Article  Google Scholar 

  9. Muir CA, Kotwal SS, Hawley CM, Polkinghorne K, Gallagher MP, Snelling P, et al. Buttonhole cannulation and clinical outcomes in a home hemodialysis cohort and systematic review. Clin J Am Soc Nephrol. 2014;9(1):110–9.

    Article  Google Scholar 

  10. Kandil H, Collier S, Yewetu E, Cross J, Davenport A. Arteriovenous fistula survival with buttonhole (constant site) cannulation for hemodialysis access. ASAIO J. 2014;60(1):95–8.

    Article  CAS  Google Scholar 

  11. National Kidney Foundation. Clinical practice guidelines for vascular access. Am J Kidney Dis. 2006;48:176–247.

    Article  Google Scholar 

  12. Labriola L, Jadoul M. Moderator’s view: buttonhole cannulation of arteriovenous fistulae; great caution is warranted. Nephrol Dial Transplant. 2016;31(4):530–3.

    Article  Google Scholar 

  13. National Kidney Foundation. Clinical practice guidelines for hemodialysis adequacy. Am J Kidney Dis. 2006;48(3):12–23.

    Google Scholar 

  14. Eknoyan G, Beck GJ, Cheung AK, Daugirdas JT, Greene T, Kusek JW, et al. Effects of dialysis dose and membrane flux in maintenance hemodialysis. N Engl J Med. 2002;347(25):2010–9.

    Article  Google Scholar 

  15. Gotch FA, Sargent JA. A mechanistic analysis of the national cooperative dialysis study (NCSD). Kidney Int. 1985;28(3):526–34.

    Article  CAS  Google Scholar 

  16. El-Sheikh M, El-Ghazaly G. Assessment of hemodialysis adequacy in patients with chronic kidney disease in the hemodialysis unit at Tanta University Hospital in Egypt. Indian J Nephrol. 2016;26(6):398–404.

    Article  CAS  Google Scholar 

  17. Rothera C, Mccallum C, Huang S, Heidenheim P, Lindsay MR. The influence of between-needle cannulation distance on the efficacy of hemodialysis treatments. Hemodial Int. 2011;15(4):546–52.

    Article  Google Scholar 

  18. Dias TS, Neto MM, da Costa JAC. Arteriovenous fistula puncture: an essential factor for hemodialysis efficiency. Ren Fail. 2008;30(9):870–6.

    Article  Google Scholar 

  19. Figueiredo AE, Viegas A, Monteiro M. Poli de Figueiredo CE. Research into pain perception with arteriovenous fistula (avf) cannulation. J Ren Care. 2008;34(4):169–72.

    Article  Google Scholar 

  20. Vaux E, King J, Lloyd S, Moore J, Bailey L, Reading I, et al. Effect of buttonhole cannulation with a polycarbonate PEG on in-center hemodialysis fistula outcomes: a randomized controlled trial. Am J Kidney Dis. 2013;62:81–8.

    Article  Google Scholar 

  21. Mac Rae JM, Ahmed SB, Atkar R, Hemmelgarn BR. A randomized trial comparing buttonhole with rope ladder needling in conventional hemodialysis patients. Clin J Am Soc Nephrol. 2012;7(10):1632–8.

    Article  Google Scholar 

  22. Santoro A. Confounding factors in assessment of delivered hemodialysis dose. Kidney Int. 2000;58:19–27.

    Article  Google Scholar 

  23. Verhallen AM, Kooistra MP, von Jaarsveld BC. Cannulation in haemodialysis: robe-ladder or buttonhole technique? Nephrol Dial Transplant. 2007;22:2601–4.

    Article  Google Scholar 

  24. Kim M-K, Kim H-S. Clinical effects of buttonhole cannulation method on haemodialysis patients. Hemodial Int. 2013;17(2):294–9.

    Article  Google Scholar 

Download references


Not applicable.


Not applicable.

Author information

Authors and Affiliations



OK, ED and AK mainly undertook this review. The literatures were screened by OK and ED. AK and OK participated in the design of the study and performed the statistical analysis. All patients underwent colour Doppler examinations by ED. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Ali Kal.

Ethics declarations

Ethics approval and consent to participate

This study was approved by the Baskent University Institutional Review Board and Ethics Committee (project no: KA18/04). The research adhered to the tenets of the Declaration of Helsinki, and a detailed written informed consent form was obtained prior to each individual’s participation in the study.

Consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kal, Ö., Duman, E. & Kal, A. Dialysis efficacy and pain levels in relation to cannulation distance using the buttonhole technique. Ren Replace Ther 6, 42 (2020).

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: